STAP細胞の懐疑点　PART52

[過去ﾛｸﾞ] STAP細胞の懐疑点　PART52 (1001ﾚｽ)
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694: 名無しゲノムのクローンさん [] 2014/03/07(金) 20:49:19.00

Despite a media frenzy, especially in Japan, with headlines even suggesting the
results are fraudulent, the authors have stood by the work. Today Niwa told
Nature News that members of the team besides Obokata have replicated the bulk
of the work and that others outside the laboratory have succeeded in the first
critical step, inducing Oct3/4 expression after the acid treatment.

But the authors admit that the procedure is more complicated than originally
advertised, leading to the publication of the tips.

The 10-page document states: “Despite its seeming simplicity, this procedure
requires special care in cell handling and culture conditions, as well as in the
choice of starting cell population.” The authors also point to the importance
of bringing the cells gradually to the brink of death ― which kills some 80% of
them after two to three days ― in order to reach the “optimal level of sub-lethal
stress”.

The tips break down the process into three sections: collection of tissue
and treatment with low-pH needed to produce STAP cells; preparing the culture
needed to convert STAP cells to STAP stem cells which behave like iPS cells or
embryonic stem cells; and preparing the culture needed to turn STAP cells into
“FI cells”, which can form placenta.

The document includes 28 “important” tips, which note the necessity of
starting with primary cells (as opposed to cultured cells) (1, ii); that mice
less than a week old, especially male mice, gave better results (1, iv); the
recommendation of using non-adhesive plates, which allow cell mobility and
cluster formation (6, i); the importance of getting the cell density right in
culture; the recommendation of using mice of a specific genetic lineage (8i),
and many other detailed hints for using the proper culture conditions.

http://uni.5ch.net/test/read.cgi/life/1394179725/694

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