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1(16): 令和大日本憂国義勇隊(栄光への開拓地) [US] [朝敵自民党から陛下の神国を護ろう！] 2022/03/12(土)22:28 AA×

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1: 令和大日本憂国義勇隊(栄光への開拓地) [US] [朝敵自民党から陛下の神国を護ろう！] 2022/03/12(土) 22:28:05.90 ID:● sssp://img.5ch.net/ico/anime_syobon02.gif Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure. https://www.mdpi.com/1467-3045/44/3/73/htm http://hayabusa9.5ch.net/test/read.cgi/news/1647091685/1

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